HomeScienceNo speed test. In esku b study bunn immunity against covid-19
No speed test. In esku b study bunn immunity against covid-19
June 10, 2020
How long does immunity to covid-19 last? How to meet a hunter with a symptom-free course? The answer to the study conducted at the University Hospital at St. Anny in Brno. The course of the study is described for Technet.cz by its leader Irena Koutn.
What exactly are you researching in your lab? N research focuses on the population names of specific CD4 + and CD8 + T-lymphocytes. These are the so-called memory beeches and, unlike nm counterparts, what memory trace the virus is able to create in the hunter’s body. They are beeches that simplify the immune response in a simplified way. This is a diameter test for any counter-pressure, which is determined, for example, by a speed test.
Our data is also important for follow-up answers, for example after shelving. Today, it is not very clear whether the vaccine will induce long-term and short-term immunity.
In the study, we want to take samples from about a hundred people who have been diagnosed with the infection and who have spilled it out, and how they are affected by their active cellular immunity to the new coronavirus. We have little to say about the role and answers of bunn immunity, so we don’t know how important the role is, how long we protect against him, how long protection lasts. Sam dleit otzky.
How does such an exam dl? From about eight milliliters of the patient’s blood, we separate the beeches, a specific population containing altered T-lymphocytes. Very simply add to the sample of the calf, more precisely a piece of one specific block of the virus. If the body encounters the virus and has produced memory cells in the virus in the T-lymphocyte spectrum, the memory cells will immediately release Interferon gamma and we will see how many you have.
km it really simplified, in fact with the patient samples, for example, let go jet, compare men, to find out the activation of other viruses.
So what exactly can you find out? Very simply, the number of cells that are replaced first against the new coronavirus. I can’t answer yes / no, but you can find out how many cells against him, how strong I can expect the immune system to respond if they encounter the virus again.
How long do such tests take? How are nron? The result is a break of hours. Some horses have to be worked for each sample separately, others in bulk, and it takes several hours to work with each test. At the same time, there are beeches in the incubator, where they mobilize, for example, or they have counter-inserts so that we can separate only the exact type of cells.
Could you do some great research to thousands of people? Yes, I think so. It’s nron, but it’s mon. We can pass 20 tests a day and we are far from the only ones who are equipped for it. It is also owned by another large hospital, to which we will of course be happy to provide instructions on how to take the test so that the staff there do not have to calibrate it, as we did with us. But they are not so full yet.
Let’s get to the current test: at what stage? Samples will be taken from Monday, May 25, and at this time we will have the results of the first patient records from the first days available. Of these, it is quite clear to see the difference between people who had the disease and who did not sell the disease. In the meantime, we do not have overlap between the two groups. For people who did not have the disease, there is basically nothing exactly measurable, all the results are on the same background. In people with a proven infection, there are many bunkers against SARS-CoV-2. There are, of course, great differences between them, practically and also one du. But there is a big difference between nakaenmi and healthy and they can be clearly distinguished from each other.
Assuming you will compare the number of memory cells with the course of the disease in patients? Yes, the participants of the study fill in a detailed questionnaire about how the disease manifested them, how they had symptoms and gave data.
Are there hospitalized patients among them, or people with a slight course? So far, we do not have samples from anyone who was really in the process and was hospitalized. Of course, we would be interested, because the patients hospitalized at our hospital had really high progress, they concentrated here the most affected by the disease. We meet these patients to get too (we took the first steps), but their convalescence is and will be nron and nevme, if only one milliliter, is somehow untouched. We have to discuss this in detail with lkai. But yes, of course we would very much like to see how cellular immunity will differ in people with different courses of disease.
So far, how many people have you studied in groups for comparison, among those who have never been positive testing? Of course, I’m interested mainly because you didn’t find any asymptomatic patients by accident. Zatm about patnct. And no, there was no one among them with whom we would be suspected of an asymptomatic contagion. Know that we would very much find someone like that. Let’s hope that someone like that could be in the control group, to which we would like to recruit a total of about 50 people.
And what about studying different age groups? I mean a specific child who, in general, has a very slight course? Of course, but it’s a question for the ethics committee. Offer the opportunity to test children from some bows, where there was a proven illness, but it is not as simple as the Dosplm mark takes about 8 and 10 milliliters, but for n such a collection is much more than for adults. Especially for young children, it is not easy, it is really a question for the ethics committee and immunologists to evaluate the benefits of such a test.
Mlo by to smysl u starch dt? I can’t answer your question at this time. The immune system especially around the age of 12 is very dynamic, with hormonal changes. It is a good question to consider whether to carry out such a study. But at this point we have to answer at least the basic questions, and then we’ll see.
Finding a hundred people with a proven contagion for research is not easy in the Czech Republic, is it? Yes, we would of course welcome the participants of the study, especially from Brno and the South Moravian Region, but there are really few of them, about 500, so yes, it’s a bit of a problem. The fact that the study on anti-inflammatory immunity also played a role also plays a role, so the interest in giving the test room is also lower among the patients, but it is still clear that new crushes are taking place every day. It is very motivating for them to find out that we are testing the memory trace of the virus. Many people understand that the answer to how strong and weak they are is first and foremost for vaccine development.
Does this mean anything for estimating the durability of this immune response? Does a small cell mean that acquired immunity will not last long, or is it not possible? In general, it cannot be assumed that my cell is bad and very good. The important thing is that they are there and they are functional. When a hunter is different, it is necessary to follow the trend. And this is exactly what we need to find out. We would like to ask our parents for an odor for about 6 msc and we will find out how the track of me remembers.
It is also important for immunologists from the point of view of their vaccine. We need to know how long the immunity of the peanut and whether you will not have to peek, and if so, how often.
st tmu for the Brno study of bunn immunity. From left Irena Koutn, Pavel imara and Lenka Tesaov.
Me studie njak pispt ik lb? This is n cl. We would very much like to get bunn lk. This means that the hunter who sold the infection would take blood and separate the T-lymphocytes inn against the coronavirus. And then we could handcuff another patient. If this is possible, let’s get a product that remembers practically an instant and is included for a very long time. Such cell therapy is also used against other viruses, such as to help patients after transplants who have an impaired immune system. This is a field that we have been working on for a long time.
But I’m a little skeptical about that. The number of memory cells specifically targeted against SARS-CoV-2 is quite low in the formulas, there are few of them, but we do not know how we can really reliably separate them. So how do we separate only that one specific type of cell and get a label that is precisely targeted, which we can be sure will actually cast and not hurt. If the patient is given other inexperienced T-lymphocytes, these would be on the body’s cells and could cause a really high-voltage problem. For a sick hunter, something like this would probably end in death.
How is tk separation and then how must it be lk ist? Specific anti-coronavirus cells make up only one fraction of the total memory cell population, which in turn makes up only one percent of the total number of cells in the sample. And in order for it to make sense, the resulting drug must, according to my estimate, contain only a per mille of unsuitable cells. We are not yet sure that this can be guaranteed. But we are a mouthful of scientific progress. And the motto of our darkness is We’ll figure it out. The team is a joint research institute of the Faculty of Medicine, Masaryk University and FNUSA, and the hospital also provides exclusive laboratories, the so-called superist premises for the production of living preparations based on beeches and tissues.
It’s a pretty nron process, too, so it’ll never be a mass creep, will it? Yes, it would probably only be for patients in a very high condition. So with cell therapies, they are quite expensive. And this only applies to those mastered techniques that are well understood and understood as the procurement of cells of the immune system, not to mention experimental procedures such as the use of stem cells. There is a situation to go much sloitj.
Even in the field of stem cells, the clinical study is slowly starting, e? Yes, but it’s about research, about experiments. And in my opinion, it is necessary to approach pluripotent stem beeches with great respect. Europe, including drug agencies, is generally relatively skeptical of them, but I understand, I even support it.
Due to the thermal treatment of stem beeches, it has become quite profaned in the last decade, and it has been about beeches from fucco, lump, or human fat, it just sounded like a time. And there was a vision of profit, and so the field of beech trunks and regenerative medicine generally gained a bad reputation.
Today the situation is different. At SKL (Sttn ad for liv control, ed.) there was a specialized resistance authorizing and monitoring this work and the EU, or its agency EMA (European Medicines Agency, pozn.red.), set clear rules. I also think that serious clinical trials will get the green light and, most importantly, that the patient will not have to worry about any inconvenience.
You have to have a lot of extra work to prepare and test. Where did you get it to ask quite simply? I liked it most of the time due to the fact that university education was dropped. Online is very easy for me, the presentation at mm always ready at the end of the semester, I also did not even need a lot of time to prepare.
Do students have pt year tit for njak lessons only online? But no, it’s not. In addition, it is good, but if I want to work, I will not change anything when it is not necessary. The scientific work is extremely interesting, it is time to see that something new and surprising is appearing below, but I still have mistakes.
Often you will receive new suggestions, students will come up with questions that the hunter would not even think of. I won’t miss that. In addition, I know histology, in which students have to look into a microscope. Online can’t be so ptt time, they can’t respond, it’s so cold.
In general, I am not a supporter of online learning. I remember the replicas of some professors in my head, and thanks to them I will also remember facts that I would not otherwise have left in my head. Life can be varied and based on different perceptions, and online learning is certainly a great one in a crisis situation, but life is outside, not behind the monitor glass.